Analysis Note

ControlEpithelial structures

Application

Anti-Epithelial Sodium Channel-α Antibody is an antibody against Epithelial Sodium Channel-α for use in WB.

Research CategoryNeuroscience

Research Sub CategoryIon Channels & TransportersNeuroinflammation & Pain

Western blot: 1-10 µg/mL using ECL and rat brain membrane preparations, see membrane preparation protocol under Technical SupportGeneral Technical InfoGeneral Methods at www.chemicon.com.

83-90kDa band is expected.

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

A 20 amino acid peptide sequence near the N-terminus of rat ENaC alpha (Linguela et al. 1993; Canessa et al.

1993; Snyder et al. 1994; Waldmann et al. 1995; Kreutz et al. 1997; Voilley et al.

1994; McDonald et al. 1994; Ludwig et al. 1998; Garty & Palmer 1997).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity Purified immunoglobulin. Liquid in phosphate buffered saline, pH 7.4, containing 0.1% BSA and 0.1% sodium azide.

ImmunoAffinity Purified

Specificity

Recognizes rat Epithelial Sodium Channel alpha (ENaC alpha). The immunogen shows no significant sequence homology with other ENaC subunits.

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

83-90kDa